Cryopreservation of human spermatozoa with minimal non-permeable cryoprotectant
نویسندگان
چکیده
منابع مشابه
On-Chip Cryopreservation: A Novel Method for Ultra-Rapid Cryoprotectant-Free Cryopreservation of Small Amounts of Human Spermatozoa
Cryopreservation of human spermatozoa free from cryoprotectant can avoid toxicity caused by highly concentrated cryoprotectant and a series of specific carriers have been previously explored, except for PDMS chip. Our study is aimed at exploring a novel device for ultra-rapid cryopreservation of small numbers of spermatozoa without cryoprotectant based on polydimethylsiloxane (PDMS) chips. Sper...
متن کاملLarge volume cryoprotectant-free vitrification: an alternative to conventional cryopreservation for human spermatozoa.
Vitrification is a simple and cost-effective method for the storage of human spermatozoa without the use of conventional cryoprotectants, by plunging the sperm suspension directly into liquid nitrogen. As a result, solidification of living cells without the formation of ice crystals is achieved during cooling. This study aimed to compare cryoprotectant-free vitrification to conventional cryopre...
متن کاملeffects of cryopreservation on plasma membrane glycoconjugates of human spermatozoa
background: cryopreservation has some detrimental impacts on sperms surface molecules. modification of the sperm surface molecules can affect on fertility rate. one of the important surface molecules are glycoconjugates. objective: the objective of this study was to evaluate the changes of content of the glycocalyx after standard cryopreservation procedure. materials and methods: forty five h...
متن کاملMembrane fluidity predicts the outcome of cryopreservation of human spermatozoa.
Semen cryopreservation is an important procedure in the treatment of human infertility. However, the ability of spermatozoa to survive freeze/thaw processes varies between patients. Cryopreservation-induced stress may result in membrane injury with consequent loss of sperm motility and viability. We investigated the relationship between the physico-chemical state of the human sperm membranes an...
متن کاملComparison of Two Cryopreservation Protocols for Freezing Human Spermatozoa
FREEZING HUMAN SPERMATOZOA Hiroshi Kobayashi, Pavithra Ranganathan, Ayman Mahran, Rakesh Sharma, Anthony J. Thomas, Ashok Agarwal, The Cleveland Clinic Foundation Objective: Sperm quality decreases significantly following freezing and research on improving cryosurvival rates is crucial. We compared the effects of two cryopreservation protocols to determine which method allows better preservatio...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cryobiology
سال: 2016
ISSN: 0011-2240
DOI: 10.1016/j.cryobiol.2016.08.004